Daniel Manduku is represented by Law Society of Kenya President Nelson Havi and Senator James Orengo.
Appearing at the Anti-Corruption Court in Nairobi, the former MD denied three charges while Mr Chigulu answered to three.
The two were granted Sh30 million bond each or Sh12 million cash bail.
The office of the Director of Public Prosecutions (DPP) has lined up at least 17 witnesses in the Sh1.4 billion corruption case facing former Kenya Ports Authority (KPA) Managing Director Daniel Manduku and works officer Juma Fadhili Chigulu.
Among the key witnesses are former KPA acting head of Ethics and Integrity Joseph Okhako and KPA Board chairman Joseph Kibwana.